Evaluation of Antiamnesic and Cholinesterase inhibitory effects of Illicium verum hook.f (Star anise) against Scopolamine induced memory impairment in Mice

Authors

  • Hafiza Tuseef Sayyar
  • Muhammad Liaquat Raza
  • Syeda Rida Baqir

DOI:

https://doi.org/10.51985/JBUMDC202151

Keywords:

Antiamnesic effect, Working memory, Reference memory, Illicium verum hook. f, Alzheimer's disease

Abstract

Objectives: The current study is carried out on the methanol extract of Illicium verum hook.f (MEIV) to evaluate and
examine anti-amnesic effects of Illicium verum hook.f by using the radial arm maze model.
Study design and setting: This experimental observational study was performed in the Department of Pharmacology,
Hamdard university from 20th November 2017 to 20th May 2018. after ethical approval from Board of Advanced studies
and Research.
Methodology: Total N=30 healthy albino mice were treated with graded doses of methanol extract of Illicium verum hook.f
(MEIV) (300 and 500mg/kg p.o.) to verify its effectiveness regarding scopolamine induce memory impairment. Afterward,
in-vitro studies were performed on isolated parts of brain mice by using an F2000 fluorescent spectrophotometer (Hitachi)
to analyze the acetylcholinesterase level.
Results: Investigation reveals that methanol extracts of Illicium verum hook.f (MEIV) by oral administration reduces the
incidence of working and reference memory errors additionally this investigation was proved that active avoidance responses in scopolamine-treated group decreased. The acetylcholine esterase level was significantly reduced at the dose of 300 mg/kg (102.05±1.93µmol/min/mg) as compared to the positive control group (161.33±2.347 µmol/min/mg).
Conclusion: Based on behavioral and biochemical investigations, we concluded that MEIV produces anti-amnesic effects
by the inhibition of acetylcholinesterase, which might be due to the presence of various essential phytochemicals such as
alkaloids, flavonoids, triterpenoid, polyphenols. Our finding reveals that Illicium verum is among the screened plants to
be assessed further as an herbal substitute for the treatment of Alzheimer's disease

References

Alikatte KL, Akondi BR, Yerragunta VG, Veerareddy PR,

Palle S. Antiamnesic activity of Syzygium cumini against

scopolamine induced spatial memory impairments in rats.

Brain and Development. 2012;34(10):844-51. doi.org/

1016/j.braindev.2012.02.008.

Megeri K. Antiamnesic evaluation of C. phlomidis Linn. bark

extract in mice. Revista Brasileira de Ciências Farmacêuticas.

;44:717-25. doi.org/10.1590/S1516-93322008000400019.

Menichini F, Tundis R, Loizzo MR. Acetylcholinesterase and

butyrylcholinesterase inhibition of ethanolic extract and

monoterpenes from Pimpinella anisoides V Brig. (Apiaceae).

Fitoterapia. 2009; 80: 297–300. doi.org/10.1016/ j.fitote.

03.008

Kaur H, Singh D, Singh B, Goel RK. Anti-amnesic effect of

Ficus religiosa in scopolamine-induced anterograde and

retrograde amnesia. Pharmaceutical biology. 2010;48(2):234-

doi.org/10.3109/13880200903271306.

Wang GW, Hu WT, Huang BK, Qin LP. Illicium verum: a

review on its botany, traditional use, chemistry and pharmacology. Journal of ethnopharmacology. 2011;136(1):10-

doi.org/10.1016/j.jep.2011.04.051.

Shahrajabian MH, Sun W, Cheng Q. Chinese star anise

('Illicium verum') and pyrethrum ('Chrysanthemum cinerariifolium') as natural alternatives for organic farming and health

care-a review. Australian Journal of Crop Science.

;14(3):517-23. doi: 10.21475/ajcs.20.14.03.p2209.

Alhajj MS, Qasem MA, Nabi AR, Al-Mufarrej SI. In-vitro

antibacterial and antifungal effects of high levels of Chinese

star anise. Brazilian Journal of Poultry Science. 2019;11:21-

doi.org/10.1590/1806-9061-2016-0427.

Wei L, Hua R, Li M. Chemical Composition and Biological

Activity of Star Anise Illicium verum Extracts Against Maize

Weevil, Sitophilus zeamais Adults. J Insect Sci 2014; 14:

–13. doi.org/10.1093/jis/14.1.80.

Vecchio MG, Gulati A, Minto C, Lorenzoni G. and: The

Multifaceted Role of Anise Plants. The Open Agriculture

Journal. 2016;10(1):30-45. doi: 10.2174/ 187433150 16100

Lee J, Nguyen QN, Park JY, Lee S, Hwang GS, Yamabe N,

Choi S, Kang KS. Protective Effect of Shikimic Acid against

Cisplatin-Induced Renal Injury: In Vitro and In Vivo Studies.

Plants. 2020;9(12):1681. doi.org/10.3390/plants9121681.

Miyagawa M, Satou T, Yukimune C. Anxiolytic-like effect

of Illicium verum fruit oil, trans-anethole and related compounds in mice. Phyther Res. Epub ahead of print 2014. doi:

1002/ptr.5190.

Nigam A, Kulshreshtha M, Panjwani D. Pharmacological

evaluation of Hibiscus abelmoschus against scopolamineinduced amnesia and cognitive impairment in mice. Advances

in Human Biology. 2019;9(2):116. doi: 10.4103/AIHB.

AIHB_3_19

Wahl D, Coogan SC, Solon-Biet SM, Cabo R, Haran JB,

Raubenheimer D, Cogger VC, Mattson MP, Simpson SJ, Le

Couteur DG. Cognitive and behavioral evaluation of nutritional

interventions in rodent models of brain aging and dementia.

Clinical interventions in aging. 2017;12:1419. doi:

2147/CIA.S145247.

El-Mahmood AM, Ogbonna OB, Raji M. The antibacterial

activity of Azadarichta indica (neem) seeds extracts against

bacterial pathogens associated with eye and ear infections.

Journal of medicinal plants research. 2013;4(14):1414-21.

doi: 10.5897/JMPR09.169.

Pitsikas N, Tarantilis PA. Effects of the active constituents of

Crocus sativus L. crocins and their combination with memantine on recognition memory in rats. Behavioural pharmacology.

;29(5):400-12. doi: 10.1007/978-3-030-62632-7_10

Du X, Choa FS, Chiappelli J, Wisner KM, Wittenberg G,

Adhikari B, Bruce H, Rowland LM, Kochunov P, Hong LE.

Aberrant middle prefrontal-motor cortex connectivity mediates

motor inhibitory biomarker in schizophrenia. Biological

psychiatry. 2019;85(1):49-59. doi: 10.1016/j.biopsych.

06.007.

Nazir N, Zahoor M, Nisar M, Karim N, Latif A, Ahmad S,

Uddin Z. Evaluation of neuroprotective and anti-amnesic

effects of Elaeagnus umbellata Thunb. On scopolamineinduced memory impairment in mice. BMC complementary

medicine and therapies. 2020 Dec;20:1-7. d Therapies (2020)

:143.doi.org/10.1186/s12906-020-02942-3

Naldi M, Brusotti G, Massolini G, Andrisano V, Temporini

C, Bartolini M. Bio-Guided Fractionation of Stem Bark

Extracts from Phyllanthus muellarianus: Identification of

Phytocomponents with Anti-Cholinesterase Activity. Molecules. 2021;26(14):4376. doi.org/10.3390/molecules26144376

Arora R, Kumar R, Agarwal A, Reeta KH, Gupta YK. Comparison of three different extracts of Centella asiatica for antiamnesic, antioxidant and anticholinergic activities: in vitro

and in vivo study. Biomedicine & Pharmacotherapy. 2018

;105:1344-52. doi.org/10.1016/j.biopha.2018.05.156.

Opara EI. Culinary herbs and spices: what can human studies

tell us about their role in the prevention of chronic noncommunicable diseases?. Journal of the Science of Food and

Agriculture. 2019 ;99(10):4511-7. doi.org/10.1002/jsfa.9658.

Pop A, Muste S, Paucean A, Chis S, Man S, Salanta L, Marc

R, Muresan A, Martis G. Herbs and spices in terms of food

preservation and shelf life. Hop Med. Plants. 2019;27:57-65.

Floyed R A, Hensley K. Aging. Neurobiology.2002;23:795-

doi.org/10.1016/S0197-4580(02)00019-2.

Soher E, Bassem A, Sabry A, Mohamed S, Shaheen B, Amal

S. Assessment of antimycotoxigenic and antioxidant activity

of star anise (Illicium verum) in vitro. J Saudi Soci of Agri

Sci. 2016;15: 20–27 . doi.org/10.1016/j.jssas.2014.05.003.

Ravi K R, Kareti S, Nargesh K. A comparative study of

polyphenolic composition and In-vitro antioxidant activity of

lllicium verum extracted by microwave and soxhlet extraction

techniques. Ind J of Pharm Educ and Rese. 2012;46(3):228-

doi.org/10.1002/ptr.989.

Li, W., Wu, Z., Xia, Y., Tan, J., Zhao. Antiviral and Antioxidant

Components from the Fruits of Illicium verum Hook.

f.(Chinese Star Anise). Journal of Agricultural and Food

Chemistry.2022; 70(12): 3697-3707.doi.org/10.1021/ acs.

jafc.1c08376.

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Published

2022-12-30

How to Cite

Sayyar, H. T. ., Raza, M. L. ., & Baqir, S. R. . (2022). Evaluation of Antiamnesic and Cholinesterase inhibitory effects of Illicium verum hook.f (Star anise) against Scopolamine induced memory impairment in Mice. Journal of Bahria University Medical and Dental College, 13(01), 08–12. https://doi.org/10.51985/JBUMDC202151

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