Effect of Methanol Extract of Brassica Oleracea on Body Weight and Hematological Parameters in Rabbits
Abstract
Objective: To evaluate the effect of methanol extract of Brassica oleracea var. capitata on body weight and hematological parameters in rabbits.
Materials and Methods: The study was conducted on 28 healthy white rabbits of either sex. All animals were equally divided into four groups. Animals of test groups were administered methanolic extract of Brassica oleracae in three different concentrations example 100, 300 and 500 mg/kg body weight respectively for 30 days. Whereas animals of normal control group received normal saline 1 ml/day equivalent to the volume of doses given to test animals. Body weights were recorded weekly on weighing machine. Hematological assay was performed twice once at 16 th day and then at the end of dosing that is at 31st day.
Results: Administration of methanolic extract of Brassica oleracae for a period of 30 days resulted in dose dependent gradual decrease in mean body weight. The reduction was significant (6% P value <0.05 ) at the dose of 300 mg/kg body weight while highly significant (14% P value < 0.01) at the dose of 500 mg/kg body weight. Hematological assay performed at 16 th day showed significant decrease in platelet count as compared to the normal control group at the doses of 300 and 500 mg/kg body weight. Whereas the decrease in platelet count was significant (P<0.05) as compared to the control group at the dose of 300 mg/kg body weight while highly significantly (P<0.01) at the dose of 500 mg/kg body weight at 31st. day on hematological examination.
Conclusion: Methanolic extract of Brassica oleracae exhibited weight reducing effect in rabbits and decreased the number of platelets in blood
References
Dahanukar S. A Kulkami R. A, Rege N N. Pharmacology of Medicinal Plants and Natural Products. Indian J. Pharm. 2000; 32:518-81
Auddy B, Ferreiro M, Blasina F, Lafon L, Arredondo F, Dajas F et al. Screening of Antioxidant Activity of Three Indian Medicinal Plants Traditionally used for the Management of Neurodegenerative Diseases. J. Eth-nopharm. 2003; 84:131-8
Jimenez- Arellanes A, Meckes M, Ramireez R, Torres J, Luna - Herrera J. Activity against multidrug-resistant mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases. J Phytother Res.2003;17: 90 -8
Raskin I, Ripoll C. Can an apple a day keep the doctor away? Curr Pharm Dec 2004; 10(27):3419-29
Gross D. Indole Phytoalexins from Brassica oleracea var. gongylodes. Planta Med, 1994, 59: A-618
Byers T, Nestle M, McTiernan A. American Cancer So-ciety 2001 Nutrition and Physical Activity Guidelines Advisory Committee. American cancer society guidelines on nutrition and physical activity for cancer prevention: Reducing the risk of cancer with healthy food choices and physical activity. CA. Cancer. J. Clin, 2002, 52: 92-119
Kris-Etherton PM, Hecker KD, Bonanome A. Bioactive compounds in foods: Their role in the prevention of car-diovascular disease and cancer. Am. J. Med, 2002;113: 71S-88S
Waqar MA, Mahmood Y. Antiplatelet, antihypercholes-terolemic and antioxidant activities of Brassica oleracea in high fat diet provided rats. World. Appl. Sci. J, 2010;8: 107-12
Jeffery EH, Araya M. Physiological effects of broccoli consumption. Phytochem Rev, 2009; 8: 283-98
Kane MP, Abu-Baker A, Busch RS. The utility of oral diabetes medications in type 2 diabetes of the young. Curr Diabetes. Rev, 2005;1: 83-92
Hasani-Ranjbar S, Jouyandeh Z, Abdollahi M J. A sys-tematic review of anti-obesity medicinal plants - an up-date. Diabetes Metab Disord, 2013 Jun 19;12(1):28. doi: 10.1186/2251-6581-12-28
Nicholson AS, Sklar M, Barnard ND, Gore S, Sullivan R, Browning S. Toward improved management of NID-DM: a randomized, controlled, pilot intervention using a low fat, vegetarian diet. Prev Med. 1999; 29: 87-91
Dickel ML, Rates SM, Ritter Mr. Plants popularly used for loosing weight purposes in Porto Alegre, South Brazil ,J Ethno pharmacol ,2007;109(1):60-71
Samec D, Piljac-Zegarac J, Bogovi M. Antioxidant po-tency of white (Brassica oleracea L. var. capitata) and Chinese (Brassica rapa L. var. pekinensis (Lour.) cab-bage: The influence of development stage, cultivar choice and seed selection. Sci Hort, 2011;128:78-83
Yakubu MT, Akanji MA, Oladiji AT. Alterations in ser-um lipid profile of male rats by oral administration of aqueous extract of Fadogia argrestis stem.Res. J.Med.Plant, 2008;2:66-73
Hossain MS, Ahmed M, Islam A. Hypolipidemic and hepatoprotective effects of different fractions of ethanolic extract of immature leaves of Mangifera indica (Linn.) in alloxan induced diabetic rats. IJPSR, 2010, 1: 132-8
National Research Council. Guide for the Care and Use of Laboratory Animals. Washington, DC: National Aca-demy Press, 1996: 1-7
World Health Organization. Nutrition. Obesity, preventing and managing the global epidemic, Report of a WHO consultation (WHO technical) Report Series 894) WHO, 2000
Popkin BM: Global nutrition dynamics: the world is shifting rapidly toward a diet linked with noncommuni-cable diseases. Am J Clin Nutr 2006, 84:289-98
Connor WE, Duell PB, Connor SL: Benefits and hazards of dietary carbohydrate. Curr Atheroscler Rep 2005, 7:428-3
Gardner CD, Coulston A, Chatterjee L. The effect of a plant-based diet on plasma lipids in hypercholesterolemic adults: a randomized trial. Ann Intern Med 2005, 142:72 5-33
Han LK, KimuraY, Okuda H. Antiobesity effects of na-tural products. Stud. Nat. Prod,chen, 2005;30:79-110
Swinburn, Caterson, Seidell, James. Diet, Nutrition and the prevention of excess weight Gain and Obesity.” Public Health Nutrition, 2004; 7(1a): 123-46
Terra X, Motagut G, Busto M, Lloplz N, Ardevol A ,Bl-ade C et al..Grape seed procyanidins prevent low grade inflammation by modulating cytokine expression in rats fed on high fat diet. J .Nutr.Biochem.2009,20:210-18
Slanc P, Doljak B, Kreft S, Luda M, Janes D, Strulelj B. Screening of selected food and medicinal plant extracts for pancreatic lipase inhibition.Phytother; Res.2009; 874-7
Harnafi H, Amrani S. Review article. Flavonoids as po-tent phytochemicals in Cardiovascular Diseases Preven-tion. Phcog rev,2007; 1:193-202
Buhler D R Miranda. Antioxidant activities of fla-vonoids. Department of Environmental and Molecular Toxicology. Oragan state of University. Linus Pauling Institute. 2000
Kang WS, Chung KH, Lee JV, Park JB, Zhang YH, Yoo HS et al. Antiplatelet activity of green tea catechins is mediated by inhibition of cytoplasmic calcium incre-ase.J.Cardiovasc.Pharmacol,2001;38:875-84
George JN.Platelets.Lancet.2000; 335:1531-9
Adebayo JO, Adesokan AA, Olatunji LA, Buoro Do, Soladoye AO. Effect of ethanolic extract of Bougainvillea Spectabilis leaves on haematological and serum lipid variables in rats.Biochem. 2005;17:45-50
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