Frequency of Urinary Tract Infections and Causative Agents in Different Age Groups in Both Genders in a Tertiary Care Hospital

Authors

  • Naseer Ahmed
  • Syed Aley-E-Hasan Zaidi
  • Salman Rasool

Keywords:

Urinary tract infection (UTI), Uropathogenic E.coli (UPEC), Midstream urine (MSU), Gram negative rods (GNR), Gram positive cocci (GPC), Pyelonephritis, Cystitis

Abstract

Objective: To determine the frequency of urinary tract infections and their causative agents in different age groups in both
genders.
Materials and Methods: All urine samples received from February 2013 to October 2013 for culture and sensitivity in Baqai
Laboratory (a subsidiary of Baqai University Hospital, Nazimabad, Karachi) were processed. Urine samples showing pyuria
were inoculated on Cysteine Lactose Electrolyte Deficient (CLED) medium and blood agar and incubated at 37OC for 24 hours.
Samples showing 105 organisms were considered to have significant bacteriuria. Organisms were identified by standard
biochemical procedures.
Results: Out of 633 samples inoculated, 40% (253) showed significant bacteriuria. Incidence of isolated uropathogens was
E.coli (70%), Enterobacter species (9%), Enterococcus species (7.5%), Psudomonas species (5%), Klebsiella species (4%),
Acinetobacter species (3.2%), Salmonella species (0.8%), Staph. aureus (less than 1%) and Candida (less than 1%). Genderwise
distribution of patients: Male 36%, Female 64%. Predominent number of female patients was in reproductive age group
while greater frequency in males was seen in patients above 55 years of age.
Conclusion: There is higher frequency of urinary infections in females in reproductive age groups and more men with urinary
infections were aged above 55. E.coli is the prominent causative organism.

References

Carr J. UTI in women: Diagnosis and management.

BMJ 2006; 332:94-7.

Seminerio JL, Aggarwal G, Sweetser S. 26 year old man

with recurrent urinary tract infection. Mayo Clin Proc

; 86(6):557-60.

Alka Neurkar. Bacterial pathogens in UTI and antibiotic

susceptibility. J Pharm Biomed Sci 2012; 21(12):1-3.

Goering R, Dockrell H, Zukerthan M. In: Mims Medical

Microbiology, 4th Ed, Mosby Elesvier; 2010 USA

.p.253-8.

Levinson W. In: Review of Medical Microbiology and

immunology 11th Edition, McGraw Hill, Lange 2010

.p.125-8.

Schaechter M, Medof G, Eisenstein BI. In: Mechanism

of Bacterial Diseases, 2nd Ed, William and Wilkins

USA 1993. p.735-47.

Collee JG, Dugid JP, Frazer A, Marmion BP, In: Mackey

and MacCartney Practical Medical Microbiology, 13th

Ed Churchil Livingstone, London 1989. p.640-7.

Qadeer N, Durrani MA, Iqbal SM. Multidrug resistant

E.coli and Klebsiella pneumoniae causing UTI in pregnant

women. Int J Pathol 2013; 116(2):45-9.

Cheesbrough M. In: District Laboratory Practic in Tropical

countries (Part 2), Cambridge University Press UK,

Reprint 2005. p.105-15.

Harvey RA, Champe PC, Fisher BD. In Microbiology

(Lippincott Illustrated Reviews) 2nd Edition, Lippincott

William and Wilkins, 530 Walnut street, Philadelphia,

PA19106, 2007. p.374-5.

Haider G, Zehra N, Munir AA, Haider A. Risk factors

of urinary tract infection in pregnancy. JPMA 2010;

(3):213-6.

Foxman B. Epidemiology of urinary tract infection:

Incidence morbidity and economic costs. Am J Med 2002; (Suppl 1A):5S-13S.

Ahmed W, Ashraf M. Isolation and antibiotic susceptibility

of gram negative bacteria associated with urinary

tract infections. Biomed Lett 2015; 1(2):70-3.

Salchev G, Pisareva E, Markova N. Virulence of uropathogenic

E.coli. J Culture Collect 2008-2009; 16:3-9.

Khan S, Ahmed A. Uropathogens and their susceptibility

pattern. A retrospective study. J Pak Med Assoc 2001;

:98-100.

Rodar JF, Alam M. Urinary tract infections. JPMA 1989;

:129-31.

Chaurasia D, Shivastava R, Shivastava S, Dubey D,

Songra M. Bacterial pathogens and their antibacterial

susceptibility. IJPBS 10, 2015; 1(1):295-8.

Ahmed SI. Antibacterial sensitivity pattern 1975-1979.

J Pak Med Assoc 1982; 32:69-71.

Farid J. Antimicrobial and clinical profile of uropathogens

at tertiary care lab. JSZMC 2013;144(2):432-4.

Mehr MT. E.coli urine superbug and its antibiotic

sensitivity. J Med Sci 2010; 18:110-3.

Sumera S. Susceptibility of uropathogen isolated from

UTI patients in tertiary care hospital. Pak J Med Sci

; 30(2):389-92.

Humayun T, Iqbal A. Culture and sensitivity pattern of

UTI in females of reproductive age. Ann Pak Inst Med

Sci 2012; 8:19-22.

Toder K. Editor, In: Todar’s Online Textbook of Bacteriology

, University of Wisconsin USA, Chapter

on E.coli .p.1-4.

Khan G, Ahmed S, Anwar S. Frequency of uropathogens

in different gender and age groups. Gomel J Med Sci

; 11:20-23.

Bien J, Sokolova O, Bozko P. Role of uripathogenic

E.coli in development of urinary tract infections and

kidney damage. Intern J Nephrol 2012; 2012:15 pages.

Article ID.681473.doi:10.1155/2012/681473

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Published

2016-09-14

How to Cite

Ahmed, N. ., Zaidi, S. A.-E.-H. ., & Rasool, S. . (2016). Frequency of Urinary Tract Infections and Causative Agents in Different Age Groups in Both Genders in a Tertiary Care Hospital. Journal of Bahria University Medical and Dental College, 6(3), 4. Retrieved from https://jbumdc.bahria.edu.pk/index.php/ojs/article/view/185

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