Effect of Nigella Sativa on Biochemical Changes in Doxorubicin- induced Nephrotoxicity in Albino Rats
Keywords:
Doxorubicin, Nigella sativa, Serum Urea, Serum CreatinineAbstract
Objective: To evaluate the protective effects of Nigella Sativa on biochemical changes in the kidney of Albino rat treated with
Doxorubicin.
Methodology: This experimental study was undertaken in April 2015 for a duration of 05 weeks at BMSI, JPMC. Forty Albino
rats were divided into four groups, ten animals in each group. Group-A was taken as control. Group-B was treated with
Doxorubicin (DOX). Group-C was given doxorubicin and Nigella Sativa (NS), and Group-D was treated with Nigella Sativa
only. At the end of the study, the serum urea and creatinine levels were measured.
Results: The mean values (mg/dl) of serum urea and creatinine levels in group-A and B were 23.40±3.07, 0.61±0.059, 85.50±7.93,
and 1.06±0.071 respectively. There was highly significant increase in the mean values of serum urea and creatinine levels in
group-B when compared with group-A. Further, the mean values (mg/dl) of serum urea and creatinine levels in group-C were
56.10±6.87 and 0.96±0.087 respectively, which showed highly significant increase when compared with group-A, and significant
decrease when these values were compared with group-B. The mean values (mg/dl) of serum urea and creatinine levels in
group-D were 25.01±3.39 and 0.67±0.057 respectively. There was insignificant increase when these values were compared with
group-A, and highly significant decrease when compared with the groups-B and C.
Conclusion: This study concludes that Doxorubicin induced biochemical changes can be minimized by the administration of
aqueous powder of Nigella Sativa. The free radical scavenging effects of Nigella Sativa might be attributed to the presence of
flavonoid, alkaloids and steroids which are powerful antioxidant and anti-inflammatory agents.
References
Soliman HAE, Ahmed RR. Gomaa HA, Ali AT. Assessment of the chemo-preventive effects of various plant constituents against doxorubicin-induced toxicity in rats. J Am Sci. 2014; 10(9):153-64
Devi KR, Kusum CL. J Pharm Life Sci. 2011; 2(11): 1195-202
Rashid S, Ali N, Nafees S, Ahmad ST, Arjuman W, Hasan SK, et al. Alleviationof doxorubicin-induced nephrotoxicity and hepatotoxicity by chrysin in Wistar rats. Toxicol Mechanisms and Methids. 2013; 23(5):337- 45
Meherzia M, Sonia H, Safwen K, Ferid L, Mohamed A, Lamjed M, et al. Grape seed and skin extract protects kidney from doxorubicin-induced oxidative injury. Pak. J. Pharm. Sci. 2016; 29(3):961-8
Hassanen MR, Mahfouz MK, Farid AS, Fadlullah AH. Biochemical effects of spirulina platensis against oxidative stress caused by doxorubicin. Benha Vet Med J 2015; 28 (2): 147-54
Chen Z, Zhu L, Zhen Y, Li D, Tang B, Chen W, et al. Anti- Proteinuric effect of Sulodexide in Adriamycin- Induced Nephropathy Rats. Latin Am J Pharm. 2012; 31(7): 963 -7
Refaie MMM, Amin EF, El-Tahawy NF, Abdel-rahman AM. Possible Protective Effect of Diacerein on Doxorubicin-Induced Nephrotoxicity in Rats. J Toxicol. 2016;2016: Article ID 9507563, 9 pages.http:// dx.doi.org/101155/2016/9507563
Quiles JL, Huertas JR, Battino M, Mataix J, Ramirez- Tortosa MC. Antioxidant nutrients and adriamycin toxicity. Toxicol 2002; 180:79-95
Mahmoud A, Mansouru HA, El-Kashef, Othman AA. Effect Of Captopril On Doxorubicin-Induced Nephrotoxicity In Normal Rats. Pharmacol Res. 1999; 39(3): 233-7
Al-Hassawi WW, Al-Sammak MA. Effect of doxorubicin on the histological structure of the kidneys in male albino rats. J Fac med Baghdad 2013; 55(4):384- 9
Al-Saedi HF, Al- Zubaidy AA, Khattab IY. The Possible Effects of Montelukast against Doxorubicin- Induced Nephrotoxicity in Rabbits. Int J Adv Res. 2014; 2(11):723-9
Ayla S, Seckin I, Tanriverdi G, Cengiz M, Eser M, Soner BC, et al. Doxorubicin Induced Nephrotoxicity Protective Effect of Nicotinamide. In J Cell Bio, 2011;2011: Article ID 390238, 9 pages http:// dx.doi.org/101155/2011/390238
Rahmani AH, Aly SM. Nigella sativa and its active constituents thymoquinone shows pivotal role in the
diseases prevention and treatment. Asian J Pharmaceu Clin. Res 2015; 8(1):48-53
Ahmad A, Husain A, Mujeeb M, Khan S, Najmi A, Siddique NA, et al. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pac J Trop Biomed. 2013;3 (5): 337-52
Habeeb AAM, El-Tarabany AA. Effect of Nigella sativa or Curcumin on daily body weight gain, feed intake and some physiological functions in growing zaraibi goats during hot summer season. Arab J Nuc Sci Appl 2012; 45(3):60-78
Hadjzadeh M, Keshavarzi Z, Yazdi S, Shirazi M, Rajaei Z, Rad A. Effect of Alcoholic Extract of Nigella Sativa on Cisplatin induced Toxicity in Rat. Iranian J of Kidney Diseases 2012; 6(2):99-104
Hosseinian S, Rad AK, Hadjzadeh M, Roshan NM, Havakhah S, Shafiee S. The protective effect of Nigella sativa against cisplatin induced nephrotoxicity in rats. Avice J Phytomed. 2016; 6(1):44-54
Mousavi G. Study on the effect of black cumin (Nigella sativa Linn.) on experimental renal ischemia- reperfusion injury in rats. Acta Cirúrgica Brasileira. 2015; 30(8):542- 9
Badary O, Abdel-Naeem A, Abdel-Wahab M, Hamada
F. The influence of thymoquinone on doxorubicin- induced hyperlipidemic nephropathy in rats. Toxicol. 2001;143:219-26
Rasha RR, Esmat AS, Hesham A, Sanaa AK. Low Dose Gamma Irradiation Modifies the Effect of L-Carnitine, Curcumin, Garlic Powder and Green tea Extract on Doxorubicin-Induced Nephropathy in Rats. Egyptian
J Hosp Med. 2010; 40: 350-64
Yusuksawad M, Chaiyabutr N. Restoration of Renal Hemodynamic and Functions during black cumin (Nigella sativa) administration in streptozotocin induced diabetic rats. J Exp pharmacol 2012;4:1-7
Dollah MA, Parhizkar S, Izwan M. Effect of Nigella sativa on the kidney function in rats. Avicenna J Phytomed 2013; 3(2):152-8
Shinde N, Jagtap A, Undale1 V, Kakade S, Kotwal S, Patil R. Protective effect of Lepidium sativum against doxorubicin-induced nephrotoxicity in rats. Res J Pharm, Bio and Chem Sci. 2010; 1(3):42
Al-Saedi HF, Al-Zubaidy AA, Khattab Yl, Sahib HB. Effect of pentoxifylline against doxorubicin induced nephrotoxicity in rabbits. Int J Pharm Sci Res 2015; 30(1):195-9
Roomi WM, Tatiana K, Nusrath WR, Matthias R, Aleksandra N. Prevention Of Adriamycin-Induced Hepatic And Renal Toxicity In Male Balb/C Mice By A Nutrient Mixture. Exp Therapeu Med. 2014; 7:1040- 44
Al-Azzawi AFS, Baraaj AH. Histological and biochemical study of Nigella sativa seeds effects on kidneys of male albino rats treated with rifampicin. World J Exp Biosci. 2016; 2: 176-80
Majeed N, Tahir M. Effect of Nigella Sativa Extract on Renal Functions in Amphotericin B Induced Nephrotoxicity in Mice. Biomedica 2014; 30(1):1-4
Onoshe S, Madusolumuo MA. Effect of aqueous seed extract of Nigella sativa on cadmium induced renal dysfunction in rats. Int J Inno App Res 2014; 2(4):1-7
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Wazeer Ahmed, Basheer Ahmed, Ashok Kumar, Mumtaz Ali
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Journal of Bahria University Medical & Dental College is an open access journal and is licensed under CC BY-NC 4.0. which permits unrestricted non commercial use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this license, visit https://creativecommons.org/licenses/by-nc/4.0 
