Correlation Between Automated And The Westergren Method For Determination
DOI:
https://doi.org/10.51985/JBUMDC2018101Keywords:
Automated Method, Erythrocyte Sedimentation Rate, Westergren Method.Abstract
Objective: To determine the correlation between automated and the Westergren method for determination of ESR in
symptomatic patients.
Study Design and Setting: This cross sectional study conducted at Hematology Department of PNS Shifa Hospital Karachi.
Methodology: Sample size was determined by using WHO sample size calculator. Westergren method was performed
according to ICSH’s specifications. ESR vacuum tubes containing sodium citrate were used. The sedimentation of the
RBC’s was recorded visually after 1 hour using a timer. Automated ESR was performed by TEST1 automated ESR analyzer
(ALIFAX SpA, Italy). It uses K3-EDTA or K2-EDTA as an anticoagulant. Bias was controlled by inclusion and exclusion
criteria and the confounding factors like temperature of lab and effect of wind were controlled by taking appropriate
measures. Pre-designed proforma was used to collect the data that was analyzed through SPSS version 16. Correlation
between the two methods was measure through Pearson correlation coefficient analysis.
Results:. The ESR measured by Westergren method ranged from 2 mm/1st hour. to 131 mm/1st hour, with a mean of
39.94±29.60 mm/1st hour. while ESR measured by automated method ranged from 2 mm/1st hour. to 120 mm/1st hr. with
a mean of 41.67±32.43 mm/1st hour. There was significantly strong correlation between automated and the Westergren
method for determination of ESR (r =0.945; p = <0.001). Similar results were observed across all ages, gender, duration
and type of symptoms groups.
Conclusion: Automated and the Westergren method are strongly correlated. Automated method can be used in place of
Westergren method for determination of ESR.
References
Danesh J, Wheeler JG, Hirschfield GM. C-reactive protein and other circulating markers of inflammation in the prediction of coronary heart disease. N Engl J Med 2004;350(14):1387-97.
Wu LA, Tan SL, Wright RS, Kopecky SL, Burrit MF, Santrach PJ, et al. Prognostic value of the erythrocyte sedimentation rate in patients with unstable angina. Arch Pathol Lab Med 2002;126(7):772.
Arikan S, Akalin N. Comparison of the erythrocyte sedimentation rate measured by the Micro Test 1 sedimentation analyzer and the conventional Westergren method'Ann Saudi Med 2007;27(5):362-5.
Ozdem S, Akbas HS, Donmez L, Gultekin M. Comparison of TEST 1 with SRS 100 and ICSH reference method for the measurement of the length of sedimentation reaction in blood. Clin Chem Lab Med 2006;44(4):407-12.
Atas A, Cakmak A, Soran M, Karazeybek H. Comparative study between the Ves-Matic and Micro erythrocyte sedimentation rate method. J Clin Lab Anal 2008;22(1):70-2.
Ibramanian A, Rangarajan K, Pandey RM, Gandhi JS, Sharma V, Bhoi SK. Evaluation of an mated erythrocyte sedimentation rate analyzer as compared to the Westergren manual method in measurement of erythrocyte sedimentation rate. Indian J Pathol Microbiol 2011;54(l):70-4.
Ajubi NE, Bakker AJ, van den Berg G. Determination of the length of sedimentation reaction in blood using the Test 1 system: comparison with the Sedimatic 100 method, turbidimetric fibrinogen levels and the influence of M proteins. Clin Chem Lab Med 2006;44(7):904-6.
Mahlangu JN, Davids M. Three-way comparison of methods for the measurement of the erythrocyte sedimentation rate. J Clin Lab Anal 2008;22(5):346-52.
Asif N, Uppal R, Mehmood S, Ahmed A, Ali S, Afzal M. Validation of automated ESR methods with conventional method as gold standard. J Islamabad Med Dent Coll 2012;2(2):81-4.
Drashti NS, Shaila NS.comparitive study of Westergren method and Automated ESR analyser(Microsed-10). JMSCR 2016;4(10):13325-29.
Kamal P,Vyankatesh TA, Shilpa S.Evaluation of micro ESR method with Westergren methodfor determination of erythyrocyte sedimentation rate. Int J Res Med Sci. 2018; 6(2):628-31
Hashemi R, Majidi A, Motamed H, Amini A, Najari F, Tabatabaey A. Erythrocyte Sedimentation Rate Measurement Using as a Rapid Alternative to the Westergren Method. Emerg (Tehran) 2015;3(2):50-3.
13Sönmez Ç, Guntas G, Kaymak AÖ, Akkaya N, Akýn KO. Comparison of Erythrocyte Sedimentation Rate Results of Test-1 and Automatic Westergren Device with Reference Westergren Method. Gazi Med J 2014;25(2):52-4
Cerutti H, Muzzi C, Leoncini R, Scapellato C, Cortelazzo A, Furlani E, et al. Erythrocyte sedimentation rate measurement by VES Matic Cube 80 in relation to inflammation plasma proteins. J Clin Lab Anal 2011;25(3):198-202.
Wiwanitkit V. Comparative study between the Westergren and automated method for determination of the erythrocyte sedimentation rate.Chiang Mai Med Bull 2001;40(3):139-41.
Horsti J, Rontu R, Collings A. Comparison between the StaRRsed Auto-Compact Erythrocyte Sedimentation Rate Instrument and the Westergren Method. J Clin Med Res 2012;2(6):261-5.
Vennapusa B, De La Cruz L, Shah H, Michalski V, Zhang QY. Erythrocyte sedimentation rate (esr) measured by the streck esr-auto plus is higher than with the sediplast westergren method. Am J Clin Pathol 2011;135(3);386-90.
AlFadhli SM, Al-Awadhi AM. Comparison of erythrocyte sedimentation rate measurement by the automated SEDIsystem and conventional Westergren method using the Bland and Altman statistical method. Med Princ Pract 2005;14(4);241-4.
Plebani M. Erythrocyte sedimentation rate: innovative techniques for an obsolete test? Clin Chem Lab Med 2003;141(2):115-6.
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Copyright (c) 2019 Hina Mushtaq, Tariq Mehmood, Imran Khan, Nabila Razvi
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