Evaluation Of Weight Changes On Wistar Rats Induced By Erythromycin And Aqueous Neem Leaves Extract

Authors

  • Nausheen Adnan
  • Uzma Bukhari
  • Iffat Raza

DOI:

https://doi.org/10.51985/JBUMDC2018088

Keywords:

Erythromycin, Hepatotoxicity, Azadirachta indica (Neem).

Abstract

Objective: To evaluate weight changes on the liver of male (albino wistar) rats induced by erythromycin. To assess the
outcome of aqueous Neem leaf extract on erythromycin induced hepatic injuries.
Methodology: Eighty male albino wistar rats were taken randomly and were divided into 4 groups of 20 animals each.
Group A as a control group. Group B rats were treated with erythromycin drug. Group C rats were treated with erythromycin
along with Aqueous Neem leaves extract. Group D were given only Aqueous Neem leaves extract. Body weight of animals
was recorded initially and also after experimentation. After 14 days animals were sacrificed, liver weight was also recorded.
Results: The liver of erythromycin treated animals group B were little bigger in size and more reddish. However erythromycin
plus Neem treated animals of group C livers were almost like liver of control group A and only Neem given group D
animals, liver were exactly same appearance like control group A.Rise in absolute and relative weight of liver was because
of drug toxicity.
Conclusion: Its established from this recent study that Aqueous Neem leaves extract being a powerful antioxidant, decreased
oxidative stress and hepatic injury caused by erythromycin

References

Harold Ellis. Anatomy of liver. Journal of surgery 2011;29(12): 589-592.

Chummy S, Sinnatam by Regional and Applied Last Anatomy, 2011;12: 238-259.

John YL Chiang. Liver Physiology: Metabolism and Detoxification. Pathobiology of Human Disease 2014, pages. 1770-1782, DOI: 10.1016/B978-0-12-386456-7.04202-7

Mitra V, Metcalf J. Functional anatomy and blood supply of the Liver. Anaesthesia & Intensive Care Medicine 2012;13(2):52-53.

Michael H. Ross, Wojciech Pawlina MD. Histology; A text and Atlas,6th edition 2015;6:628-658.

Poisson J, Lemoinne S, Boulanger C, Durand F, Moreau R, Valla D, Rautou PE. Liver sinusoidal endothelial cells: Physiology and role in liver diseases. Journal of Hepatology 2017;66(1): 212–27.

Mohamed M, Abdel–Daim. Pharmacodynamic interaction of Spirulina platensis with erythromycin in Egyptian Baladi bucks (Capra hirus). Journal of research sciences 2014;120(2-3):234-241.

Bosnjakovic A, Manoj K, Ren W, Kurtoglu YE, Shi T. poly (amidoamine) dendrimer -erythromycin conjugates for drug delivery to Macrophages involved in periprosthetic inflammation. Journal of nanomedicine; Nanotechnlogy, Biology and medicine. 2011; (7):

Sambo N, Garba SH and Timothy H .Effect of aqueous extract of Psidium Guajava on erythromycin induces Lives Damage in Rats. Nigerian Journal of Physiology sciences. 2009; 24(2): 171-176.

Chhibber, S, Sharma, N. Medicinal and therapeutical potential of Neem (azadirachta indica).International Journal of Scientific and Research Publications 2014; 4(5):2250-3153.

Girish K, Bhat S. Neem –green treasure .Electronic Journal of Biology 2008; 4930(10); 102-111.

. Singh V, Chauhan D. Phytochemical evaluation of aqueous and ethanolic extract of neem leaves (Azadiractica indica) Indoamerican journal of pharmaceutical research .2014; 4(12):43-48.

Mohammad A .Alzohairay .Therapeutics Role of Azadirachta indica (neem) and their Active constituents in Disease Prevention and treatment. Evidence Based Complementary and Alternative Medicine .2016; 10(7): 1-11.

Rao Chava V, Manjunath SM, Rajanikanth AV, Sridevi N. The efficacy of Neem Extract on four Microorganisms Responsible For causing Dental Caries viz Streptococcus mutans,Streptococcus salivarius, Stretococcus mitis and Streptoccocus sanguis.The journal of Contemporary Dental practice 2012; 13(6):769-772.

Sunday O. Okoh, Ade O. Oyewole1, Ruth O. Ishola1, Adenike D. Odusote1, Omobola O. Okoh, Chima C. Igwe1 & Gloria N. Elemo. Antioxidant and Free Radical Scavenging Capacity of Crude and Refined Oil Extracted from Azadirachta indica A. Juss International Journal of Biology 2015;7(2):78-82.

Baligar NS, Aladakatti RH, Ahmed M, Hiremath MB. Hepatoprotective activity of neem based constituent azadirachtin –A, in carbon tetrachloride intoxicated Wistar rats journal physiol pharmacol 2014; 92(4): 67-77.

Nassr-Allah H, Abdel-Hameid .Protective role of dimethyl diphenyl bicarbooxylate (DDB) against erythromycin induced hepatotoxicity in male’s rats. Journal of toxicology in vitro 2007; 10: 121-130.

Mansi Y, Abdelaziz N. Ezzeldin Z. Ibrahim R .Randomized controlled trial of a high dose of oral erythromycin for the treatment of feeding intolerance in preterm infants. Neonatology. 2011;100(3): 290-294.

David E. Amacher, Schomaker SJ, Retsema JA. Comparison of the Effects of the New Azalide Antibiotic, Azithromycin, and Erythromycin Estolate on Rat Liver Cytochrome P-450. Antimicrobial agents and Chemotherapy 1991:35(6):1186-90.

John AA, Bamidele FP and OluwaSeun OA. Some Protective Effects of Aqueous Leaf Extract of Azadirachta indica on Paracetamol-induced Hepatotoxicity in Adult Wistar Rats. American Journal of tropical medicine & Public Health 2011;1(3): 97-106,

Robert R. Maronpot, Yoshizawa K, Nyska A, Harada T, Flake G, Mueller G, Singh B, Ward JM. Hepatic Enzyme Induction: Histopathology. Toxicologic Pathology, 2010;38:776-795.

Dkhil MA, Al-Quraishy S, Aref AM, Othman MS, Kamal M. El-Deib and Moneimet AEA. The potential role of azadirchtica Indica treatment on cisplatin induced hepatotoxicity and oxidative stress in female Rats. Oxidative Medicines and cellular longevity. 2013;741817, pages 9. http://dx.doi.org/ 10.1155/2013/741817

Oluwole B. Akinola, Oluwafunmike S. Akinolaet, Ezekiel A. Intestinal lesions of the streptozotocin-induced diabetes and the effects of Azadirachta indica treatment 2009. Pharmacologyonline 3: pages, 872-881.

ChavanT, GhadgeA, KarandikarM, PanditV, RaniekarP, KulkaeniO, KuvalekarA, MantriN. Hepatoprotective Activity of Satwa, an Ayurvedic Formulation, Against Alcohol-induced Liver Injury in Rats Altern Health Med. 2017; 23(4): 34-40.

Robert R. Maronpot, Yoshizawa K, Nyska A, Harada T, Flake G, Mueller G, Singh B, Ward JM. Hepatic Enzyme Induction: Histopathology. Toxicologic Pathology, 2010;38:776-795

XiaoyanLu, TianY, LianX, Ji, jinT, ZhaoQ, HuB, ShenY, fanX, Integrated system toxicology approaches identified the possible involment of ABC transporters pathway in erythromycin Esolate-induced liver injury in rats. Food and Chemical toxicology 2014;65(6): 343-355

Nassr-Allah H, Abdel-Hameid .Protective role of dimethyl diphenyl bicarbooxylate (DDB) against erythromycin induced hepatotoxicity in male’s rats. Journal of toxicology in vitro. 2007; 10: 121-130.

RB Raizada, M.K. Srivasta, R.A. Kaushal, R.P.Kaushal. Azadiractica indica .A Neem pesticide subchronic toxicity assessment in rats. Food and chemical Toxicology 2001; 39(7): 477-483.

Innih, Silvanus Olu, EZE. The effect of Aqueous Extract of Aqueous Neem (Azadirachtica Indica) leaves on liver functions of wistar rats. A Peer-review Journal of Biomedical Sciences. 2014; 13(2):61-66.

Ashafa AOT, Orekoya LO, Ykubu MT. Toxicity profile of Ethanolic extract of Azadiractica indica stem bark in male wistar rats. Asian Pacific Journal of tropical Biomedicine 2012; 2(10): 811-817.

Ezz-Din D, Gabry MS, Farrag ARH and Moneim AEA. Physilogical and histological impact of azadirachtica indica extract in a rat model of cisplastin induced hepato and nephrotoxicity. Journal of Medicinal Plants Research 2011; 5(23): 5499-5506.

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Published

2019-03-05

How to Cite

Adnan, N. ., Bukhari, U. ., & Raza, I. . (2019). Evaluation Of Weight Changes On Wistar Rats Induced By Erythromycin And Aqueous Neem Leaves Extract. Journal of Bahria University Medical and Dental College, 9(1), 03–07. https://doi.org/10.51985/JBUMDC2018088

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