Evaluation of Neurobehavioral Effect of Citrullus Lanatus Juice on Albino Mice
Keywords:
Anti-anxiety effect, Anti-depressant effect, Citrullus lanatus, Diazepam, FluoxetineAbstract
Objective: To observe the effect of Citrullus lanatus var. lanatus on albino mice model of anxiety and depression.
Methodology: This experimental study was done in the Pharmacology department of Karachi University for a period of 02
months from 5th February-9th April 2016. Watermelon (Citrullus lanatus var. lanatus) was used. Animals were randomly assigned
into 4 groups A, B, C and D (n=10). Group A was control (normal saline). Group B was treated with Citrullus lanatus juice in
a dose of 3 ml/kg, Group C was given 6 ml/kg of juice. Two drugs (Diazepam and Fluxetin) were used as standard and given
to group D animals. All doses were administered orally. Level of anxiety and depression among animals was tested by light/dark
box test and forced swim test. Data was analyzed statistically.
Results: Animals treated with Citrullus lanatus juice showed marked reduction in anxiety which was evident from results of
light/dark box test while forced swim test revealed no effect on depression.
Conclusion: Citrullus lanatus contains important natural anti-oxidants like lycopene and vitamin C which prevent injury to
vital organs like liver, heart and brain from toxic effects of reactive oxygen species. Arginine in Citrullus juice is also precursor
of neurotransmitters like -Aminobutyric acid (GABA) and glutamate. These results showed that Citrullus because of its
components like arginine played an important role in maintaining normal neurological function and ameliorated anxiety and
depression.
References
Ghorpade P, Siddiqui A, Patil MJ, Rub RA. Pharmaco- gnostic and phytochemical evaluation of Celosia argentea. Pharmacognosy Journal. 2012 Dec 1;4(33):7-15
Gorinstein S, Yamamoto K, Katrich E, Leontowicz H, Lojek A, Leontowicz M et al. Antioxidative properties of Jaffa sweeties and grapefruit and their influence on lipid metabolism and plasma antioxidative potential in rats. Bioscience, biotechnology, and biochemistry. 2003 Jan 1;67(4):907-10
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African journal of biotechnology. 2005;4(7):685-8
Johnson JT, Iwang EU, Hemen JT, Odey MO, Efiong EE, Eteng OE. Evaluation of anti-nutrient contents of watermelon Citrullus lanatus. Ann Biol Res. 2012; 3 (11):5145-50
Rolls BJ, Ello-Martin JA, Tohill BC. What can intervention studies tell us about the relationship between fruit and vegetable consumption and weight management? Nutrition reviews. 2004 Jan 1;62(1):1-7
Erhirhie EO, Ekene NE. Medicinal values on Citrullus lanatus (Watermelon): Pharmacological review. Intern- ational Journal of Research in Pharmaceutical and Bio- medical Sciences. 2014;4(4):1305-12
Edwards AJ, Vinyard BT, Wiley ER, Brown ED, Collins JK, Perkins-Veazie P, et al. Consumption of watermelon juice increases plasma concentrations of lycopene and n-carotene in humans. The Journal of nutrition. 2003 Apr 1;133(4):1043-50
Leskovar D, Bang H, Crosby K, Maness N, Franco A, Perkins-Veazie P. Lycopene, carbohydrates, ascorbic acid and yield components of diploid and triploid watermelon cultivars are affected by de® cit irrigation. The Journal of Horticultural Science and Biotechnology. 2004 Jan 1; 79(1):75-81
Hong MY, Hartig N, Kaufman K, Hooshmand S, Figueroa A, Kern M. Watermelon consumption improves inflam- mation and antioxidant capacity in rats fed an atherogenic diet. Nutrition Research. 2015 Mar 31; 35(3):251-8
Fu WJ, Haynes TE, Kohli R, Hu J, Shi W, Spencer TE, et al. Dietary L-arginine supplementation reduces fat mass in Zucker diabetic fatty rats. The Journal of Nutrition. 2005 Apr 1; 135(4):714-21
Jamuna KS, Ramesh CK, Srinivasa TR, Raghu KL. In vitro antioxidant studies in some common fruits. Int J Pharm Pharm Sci.2011; 3(1): 60-3
Williams K. Interactions of polyamines with ion channels. Biochemical Journal. 1997; 325(2):289-97
Karim N, Irshad S, Khan I, Mohammad A, Anis I, Shah MR. GABA A receptor modulation and neuropharma- cological activities of viscosine isolated from Dodonaea- viscosa (Linn). Pharmacology Biochemistry and Behavior. 2015; 136: 64-72
Eisenberg DM, Davis RB, Ettner SL, Appel S, Wilkey S, Van Rompay M, et al. Trends in alternative medicine use in the United States, 1990-1997: results of a follow- up national survey. JAMA. 1998 Nov 11; 280(18):1569-75
Calhoon GG, Tye KM. Resolving the neural circuits of anxiety. Nature Neuroscience. 2015 Oct 1; 18(10):1394
Azmat A, Ahmed M, Haider S, Haleem DJ, Syed NZ, Ahmad I. Neuropharmacological profile of somina (a poly herbal unani formulation) in rats and mice. Nigerian Journal of Natural Products and Medicine. 2005;9(1):48- 52
Möhler H. The GABA system in anxiety and depression and its therapeutic potential. Neuropharmacology. 2012 Jan 31;62(1):42-53
Shen Q, Lal R, Luellen BA, Earnheart JC, Andrews AM, Luscher B. -Aminobutyric acid-type A receptor deficits cause hypothalamic-pituitary-adrenal axis hyperactivity and antidepressant drug sensitivity reminiscent of melancholic forms of depression. Biological psychiatry. 2010 Sep 15; 68(6):512-20
Oyewo OO, Onyije FM, Akintunde OW, Ashamu EA. Effects of aqueous extract of Citrullus lanatus on the histology of the kidney of adult Wistar rats. World Applied Sciences Journal. 2012;17(9):1178-81
Bhattamisra SK, Singh PN, Singh SK, Kumar V. Anxiolytic activity of Marsileaminuta Linn in rodents. Journal of Herbal Medicine and Toxicology. 2007; 1(1):15-20
Kwon S, Lee B, Kim M, Lee H, Park HJ, Hahm DH. Antidepressant-like effect of the methanolic extract from Bupleurum falcatum in the tail suspension test. Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2010 Mar 17; 34(2):265-70
Kumar M, Shete A, Akbar Z.A Review on Analgesic: From Natural Sources. International Journal of Pharma- ceutical & Biological Archives. 2010; 1(2): 95– 100
Yativ M, Harary I, Wolf S. Sucrose accumulation in watermelon fruits: genetic variation and biochemical analysis. Journal of plant physiology. 2010 May 15; 167 (8):589-96
Mishra SK, Singh PN, Dubey SD. Evaluation of CNS depressant activity of Capparis Zeylanica Lin. root. Research Journal of Medicinal Plant. 2011; 5(6):738-46
Dolai N, Karmakar I, Kumar RB, Haldar PK. CNS depre- ssant activity of Castanopsis indica leaves. Oriental Pharmacy and Experimental Medicine. 2012 Jun 1;12 (2):135-40
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