Restorative Effect of Garlic on Fatty Liver
Keywords:
Fatty liver, Garlic, High-fat diet, Hepatocellular damage, Saturated fats.Abstract
Objective: To assess the restorative role of garlic on high-fat induced hepatocellular damage.
Materials and Methods: This experimental study was done at BMSI, JPMC from 1st - 30th October 2008 after obtaining ethical
approval. Thirty adult albino rats, weighing 220 to 240 gram were divided into three groups according to dietary regimen. Group
A served as control, group B received high-fat diet (20 mg butter in 100 gm of diet) and group C was given same quantity of
butter as in group B along with 6% fresh crushed garlic in diet for 4 weeks. The rats were sacrificed, liver removed, weighed
and processed for haematoxylin and eosin staining.
Results: There was significant increase in the body weight and absolute liver weight in group B animals receiving high-fat diet,
both of which decreased significantly after the concomitant use of garlic in group C. Haematoxylin and eosin stained sections
revealed shrunken portal triad, swollen hepatocytes with pyknotic nuclei in high-fat diet group as compared to control animals,
while in group C hepatocytes were polygonal in shape with vesicular nuclei comparable to control group A.
Conclusion: Garlic has restorative role in high-fat induced hepatocellular damage. Fatty liver produced by high quantities of
saturated fats, such as butter can be ameliorated by the use of garlic.
References
Fan JG, Jia JD, Li YM. Guidelines for the diagnosis and
management of nonalcoholic fatty liver disease: update.
Chinese Journal of Hepatology 2010; 18:163-6.
Chalasani N, Younossi Z, Lavine JE. The diagnosis and
management of non-alcoholic fatty liver disease: practice
guideline by the American Gastroenterological Association,
American Association for the Study of Liver Disease
Gastroenterology. Gastroenterology 2012; 142: 1592-
Carthy EM, Rinella ME. The role of diet and nutrient
composition in nonalcoholic Fatty liver disease. J. Acad.
Nutr. Diet.2012; 112: 401-9.
Mouzaki M, Allard JP. The role of nutrients in the development,
progression, and treatment of nonalcoholic fatty
liver disease. J. Clin. Gastroenterol. 2012; 46: 457-67.
Carvalhana S, Machado MV, Cortez-Pinto H. Improving
dietary patterns in patients with nonalcoholic fatty liver
disease. Curr Opin Clin Nutr Metab. Care 2012; 15:
-73.
Hartvigsen K, Binder CJ, Hansen LF. A diet-induced
hypercholesterolemic murine model to study atherogenesis
without obesity and metabolic syndrome. Arteriosclerosis,
Thrombosis and Vascular Biology.2007; 27:
-85.
Sobenin IA, Andrianova IV, Fomchenkov IV, Gorchakova
TV, Orekhov AN. Time-released garlic powder tablets
lower systolic and diastolic blood pressure in men with
mild and moderate arterial hypertension. Hypertens Res
;32:433-7.
Xiao J, Liong EC, Ling MT. S-allylmercaptocysteine
reduces carbon tetrachloride-induced hepatic oxidative
stress and necroinflammation via nuclear factor kappa
B-dependent pathways in mice. Eur J Nutr 2012; 51:
-33.
Mahan LK, Arlin MT. Krause's Food, Nutrition and Diet
Therapy. 8th ed. Philadelphia: W.B. Saunders Company,
.p.726-7.
Woods SC, Seeley RJ, Rushing PA, D'Alessio D, Tso
P. Controlled High-fat Diet Induces an Obese Syndrome
in Rats. J Nutr 2003; 133: 1081-7.
Qureshi AA, Abuirmeileh N, Din ZZ. Inhibition of cholesterol
and fatty acid biosynthesis in liver enzymes and
chicken hepatocytes by polar fractions of garlic. Lipids
; 18: 343-8.
Bancroft JD, Gamble M. Theory and practical of Histological
Techniques. 6th ed. Edinburgh: Elsevier Churchill
Livingstone, 2008.p. 25-7, 134-5, 177-8.
Adams LA, Sanderson S, Lindor KD, Anguld P. The
histological course of nonalcoholic fatty liver disease:
a longitudinal study of 103 patients with subsequent
liver biopsies. Journal of Hepatology, 2005; 42 (1): 132-8.
Elsayed HH, Elrahman MK, Emara AH, El-Hafez AA.
Compare Effect of fatty acid Composition (Olive oil,
Coconut Oil and Butter) on Adipose Liver tissue, and
Serum Lipid Profile in Albino Rats. IOSR Journal of
Biotechnology and Biochemistry, 2015; 1(3): 28-38.
Yassin ME, Haggag E, Elsanhoty RM, Ramadan MF.
Impact of dietary oils and fats on lipid peroxidation in
liver and blood of albino rats. Asian Pacific Journal of
Tropical Biomedicine, 2014; 4(1): 52-8.
Brunt EM. Pathology of fatty liver disease. Modern
Pathology, 2007; 20: 40-8.
Bloomgarden ZT. Relationship between insulin resistance
and nonalcoholic fatty liver disease, and aspects of
insulin resistance in children and adolescence. Diabetes
Care, 2007; 30 (6): 1663-9.
Aller R, Izoala O, Fuente B, Luis D. Mediterranean diet
is associated with liver histology in patients with nonalcoholic
fatty liver disease. Nutr Hosp, 2015; 32 (6):
-24.
Qamar A, Siddiqui A, Kumar H. Fresh garlic amelioration
of high-fat-diet induced fatty liver in albino rats. Journal
of Pakistan Medical Association, 2015; 65: 1102-7.
Fauci AS, Kasper DL, Longo DL, Braunwald E, Hauser
SL, Jameson JL, Loscalzo J: Harrison’s Principles of
Internal Medicine. 17th ed., New York, McGraw-Hill
Companies, 2008 .p.1982-83.
Jen KC, Buison A, Pellizzon M, Ordiz F, Ana LS, Brown
J. Differential effects of fatty acids and exercise on body
weight regulation and metabolism in female wistar rats.
Experimental Biology and Medicine, 2003; 228: 843-9.
Yeh Y, Liu L. Cholesterol-lowering effects of garlic
extracts and organosulfur compounds: human and animal
studies. Journal of Nutrition; 2001:131: 9895-938.
Bari O, Neuschwander-Tetri BA, Liu M, Portincasa P,
Wang DQH. Ezetimibe: Its novel effects on the prevention
and the treatment of cholesterol gallstones and
nonalcoholic fatty liver disease. Journal of Lipids, 2012;
Article ID 302847, 16 pages.
Rajasree CR, Rajmohan T, Augusti KT. Antiatherogenic
and antiperoxidative effects of garlic and soy proteins
in alcohol fed rats. Indian Journal of Experimental
Biology 2008; 47: 1169-75.
Lieber CS, Leo MA, Mak KM, Xu Y, Cao Q, Ren C et
al. Model of Non-alcoholic Steatohepatitis. American
Journal of Clinical Nutrition 2004; 79: 502-9.
Das D, Mukherjee M, Das AS, Mukherjee S, Mitra C.
Aqueous extract of black tea (Camellia sinensis) prevents
chronic ethanol toxicity. Current Science, 2005; 88: 952-61
Xiao J, Guo R, Fung M, Liong EC, Chang RCC, Ching
Y et al. Garlic-Derived S-Allylmercaptocysteine
Ameliorates Nonalcoholic Fatty Liver Disease in a Rat
Model through Inhibition of Apoptosis and Enhancing
Autophagy. Evidence-Based Complementary and
Alternative Medicine 2013; article ID 642920: 11 pages.
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Copyright (c) 2016 Aisha Qamar, Humera Waqar, Mohammad Saad Saeeduddin
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