Estimation of Oxidant and Antioxidant Levels in Hemodialysis Treated end Stage Renal Disease Patients and Comparison with Normal Population

Abstract

Objectives: To estimate the plasma oxidant and antioxidant levels in hemodialysis treated end stage renal disease patients
and to match them with normal population.
Study Design and Setting: It was a comparative prospective study. This study was carried out at Jinnah Post Graduate
Medical Center Karachi, from January 2018 till December 2018.
Methodology: The hemodialysis patients (group B) were selected from the Dialysis Center of JPMC Karachi whereas the
controls (group A) were recruited from normal healthy population. Consecutive sampling technique was used. The cases
taken were receiving maintenance hemodialysis thrice a week. Antioxidant levels were determined by estimating plasma
superoxide dismutase (SOD) while oxidant levels were estimated by detection of serum malondialdehyde (MDA) and
compared with the levels of control group.
Results: The mean superoxide dismutase level in control group was 108.53±19.44 while mean SOD levels in hemodialysis
group was 46.20±19.18. . In control group mean MDA was 10.87±3.04, and in hemodialysis group mean MDA was
31.01±8.48.This results show the increased risk of oxidative stress resulting in complications in hemodialysis patients.
Conclusion: Antioxidant levels are reduced in hemodialysis patients as compared to the normal population while the
oxidant levels are much increased in hemodialysis group. This imbalance contributes to the oxidative stress related
complications taking place in these patients. This study will help the nephrologist to elaborate the protective role of
antioxidant administration in oxidative stress that can improve the cardiovascular mortality rate in hemodialysis treated
end stage renal disease.